PCR Vs Vector Cloning

Vector Cloning

1. Relatively cheaper compared to PCR
2. Replicates entire DNA (recombinant vector)
3. Requires bacteria and plasmid (vector)
4. Required or desired DNA is inserted in the plasmid and produce desired protein
5. Requires specific restriction enzymes
6. Has practical uses in treating medical disorder (somatropin for stunted growth)

PCR (polymease chain reaction)

1. Requires less time
2. Three step cycle: heating, cooling, and replication
3. Higher probability of damage or corruption. If only one DNA is replicated improperly, every DNA strand in that set will be damaged as well.
4. Only replicates DNA but cannot produce protein
5. Useful for forensics
6. Medical uses more centered in help with diagnosis (of HIV)